Cyclooxygenase (COX) Activity Assay Kit (Fluorometric) (ab204699) (2024)

Overview

• Product name

  Cyclooxygenase (COX) Activity Assay Kit (Fluorometric)

• Detection method

  Fluorescent

  See Also

  GERD and LPR: How to Tell, Flares, Remedies, AntacidsAntiulcer and gastroprotective potential of Stereospermum suaveolens in Wistar RatsEstimates of all cause mortality and cause specific mortality associated with proton pump inhibitors among US veterans: cohort study

• Sample type

  Tissue, Adherent cells, Suspension cells

• Assay type

  Enzyme activity

• Assay time

  0h 45m

• Species reactivity

  Reacts with: Mammals, Other species
  

• Product overview

  CyclooxygenaseCOX Activity AssayKit (Fluorometric) (ab204699)provides a simple, sensitive, and high-throughput adaptable method to detect the peroxidase activity of COX in biological samples orpurified/crude enzyme preparations. The kit includes COX-1 and COX-2 specific inhibitors to differentiate the activity of COX-1 and COX-2as well as other peroxidases, which may be present in the sample. Detection limit: 6 μU/mg.

  
  

  Cyclooxygenase assay protocol summary:
  - add samples and standards to wells
  - add reaction mix
  - add arachidonic acid/NaOH solution
  - analyze with microplate reader every 15 s for 30 min

• Notes

  This product is manufactured by BioVision, an Abcam company and was previously called K549 Cyclooxygenase (COX) Activity Assay Kit (Fluorometric). K549-100 is the same size as the 100 test size of ab204699.

  Cyclooxygenase (COX), also known as prostaglandin-endoperoxide synthase (PTGS, EC 1.14.99.1), is an enzyme that is responsible forthe formation of important biological mediators called prostanoids, including prostaglandins, prostacyclin and thromboxane. COX is thecentral enzyme in the biosynthetic pathway to prostanoids from arachidonic acid. There are two known isoenzymes: COX-1 and COX-2.COX-1 is constitutively expressed in many tissues and is the predominant form in gastric mucosa and in kidney. COX-2 is not expressedunder normal conditions in most cells, but elevated levels are observed during inflammation. Pharmacological inhibition of COX by nonsteroidalanti-inflammatory drugs (NSAID) can provide relief from the symptoms of inflammation and pain.

• Platform

  Microplate reader

Properties

• Storage instructions

  Store at -20°C. Please refer to protocols.

• Components	100 tests
  Arachidonic Acid	1 vial
  Celecoxib (COX-2 Inhibitor)	1 x 100µl
  COX Assay Buffer	1 x 25ml
  COX Cofactor	1 x 20µl
  COX-1 Positive Control	1 vial
  NaOH Solution	1 x 500µl
  OxiRed Probe	1 x 200µl
  Resorufin Standard	1 x 50µl
  SC560 (COX-1 Inhibitor)	1 x 100µl

• Research areas

  • Neuroscience
  • Neurology process
  • Neurodegenerative disease
  • Other

  • Cardiovascular
  • Atherosclerosis
  • Ischemia / Reperfusion

  • Neuroscience
  • Processes

• Cyclooxygenase (COX) Activity Assay Kit (Fluorometric) (ab204699).

  Typical Resorfurin Standard calibration curve.

• Cyclooxygenase (COX) Activity Assay Kit (Fluorometric) (ab204699).

  Measurement of COX-1 activity in absence (blue line) and presence of COX-1 inhibitor (SC560, red line) using the COS-1 positive control (PC) provided in the kit.

• Cyclooxygenase (COX) Activity Assay Kit (Fluorometric) (ab204699).

  Detection of endogenous COX-1 activity in J774 cell lysate (6 μg) and rat liver hom*ogenate (210 μg).

• Cyclooxygenase (COX) Activity Assay Kit (Fluorometric) (ab204699).

  Detection of endogenous COX-2 activity in J774 cell lysate (7 μg) stimulated with or without 100 ng/ml LPS and 100 ng/ml murine IFN-gamma.

• Protocol Booklet

Click here to view the general protocols

• SDS download

  Country/Region

  Language

  

• Datasheet download

  Download

ab204699 has been referenced in 8 publications.

• Grande F et al. Toward Multitasking Pharmacological COX-Targeting Agents: Non-Steroidal Anti-Inflammatory Prodrugs with Antiproliferative Effects. Molecules 26:N/A (2021). PubMed: 34203324
• Wang J et al. Betalain exerts a protective effect against glaucoma is majorly through the association of inflammatory cytokines. AMB Express 10:125 (2020). PubMed: 32666339
• Mauri E et al. Ester coupling of ibuprofen in hydrogel matrix: A facile one-step strategy for controlled anti-inflammatory drug release. Eur J Pharm Biopharm 146:143-149 (2020). PubMed: 31726217
• Montaser A et al. Astrocyte-Targeted Transporter-Utilizing Derivatives of Ferulic Acid Can Have Multifunctional Effects Ameliorating Inflammation and Oxidative Stress in the Brain. Oxid Med Cell Longev 2019:3528148 (2019). PubMed: 31814871
• Guenzle J et al. Cyclooxygenase (COX) Inhibition by Acetyl Salicylic Acid (ASA) Enhances Antitumor Effects of Nitric Oxide in Glioblastoma In Vitro. Mol Neurobiol N/A:N/A (2019). PubMed: 30715649
• Gilman KE et al. P2X7 receptor deletion suppresses ?-radiation-induced hyposalivation. Am J Physiol Regul Integr Comp Physiol 316:R687-R696 (2019). PubMed: 30892913
• Destefanis F et al. Investigating the Connection Between Endogenous Heme Accumulation and COX2 Activity in Cancer Cells. Front Oncol 9:162 (2019). PubMed: 30941311
• Aytaç PS et al. Novel triazolothiadiazines act as potent anticancer agents in liver cancer cells through Akt and ASK-1 proteins. Bioorg Med Chem 24:858-72 (2016). PubMed: 26810835